Y04603 Recombinant Human Wnt-3a(hFc Tag）

 

Recombinant Human Wnt-3a(hFc Tag）

Catalog Number: Y04603

 

Characteristics:

Source                                         HEK293

Description                                 Human Wnt-3a (Ser19-Lys352) with a C-terminal linker GGGGGS and Human lgG1 (Asp104-                                                         Lys330)

                                                     Accession # NP_149122

Predicted molecular mass        58.2kDa

 

Specification:

Appearance

White powder,Colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining)

Endotoxin

≤100 EU/mg by the LAL method

Activity

Measured by its ability to induce Topflash reporter activity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml..

Formulation

Lyophilized from a 0.2 um filtered solution containing PBS, pH7.4

Size

50 μg/vial, 1 mg/vial

 

Handling and Storage:

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice

Storage & Stability

36 months at 2°C to 8°C in lyophilized state

6 months at -20°C to -80°C under sterile conditions after reconstitution

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.