T01001 Recombinant human TIM1(His Tag)

产品信息(Information)：

背景介绍(Background)：

The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4, 12 and 19. [provided by RefSeq, Apr 2015].

T cell immunoglobulin and mucin domain-1 (TIM-1), a mucin-like protein is mainly expressed in Th2 cells. It acts as a costimulatory molecule for T cell activation and effector functions, also acts as a major P-selectin ligand on T cells. The extracellular portion of human TIM proteins is highly conserved, with an N-terminal IgV-like domain adjacent to a mucin-like domain. The latter is glycosylated to different degrees in different TIM proteins, with TIM-1 and TIM-4 bearing the most glycan chains (57 and 41 predicted O-glycosylation sites respectively). The intracellular tails of TIM-1 and TIM-3 also contain at least one tyrosine-kinase phosphorylation motif, whereas TIM-4 does not mediate direct transmembrane signals. TIM proteins also contain a phosphatidylserine-binding site in the IgV-like domain.

Several lines of evidence indicate that TIM-1 has the broadest range of immune functions among the TIM proteins. TIM-1 is a master regulator of immune responses and acts as a trafficking receptor for effector T cells. TIM-1 belongs to the restricted group of major adhesion receptors involved in leukocyte tethering and rolling under inflammatory conditions. TIM-1 is present in large intracellular pools in the Golgi apparatus and endoplasmic reticulum (ER), in early endosomes (EE), and in lysosomal compartments. TIM-1 is targeted to the plasma membrane through exocytosis vesicles (EV) released by the Golgi apparatus. The EVs can fuse with lysosomes explaining the role of TIM-1 in the degradation of intracellular proteins. TIM-1 continuously undergoes retrograde trafficking through clathrin-dependent endocytosis followed by fusion with the transGolgi network. TIM-1 is also detected on the cell membrane, where it is presumably anchored in a bent conformation to phosphatidylserine (PS) in the inner leaflet through the MILIBS motif in the IgV-like domain. When T cells are activated and polarized toward T helper cell 1 (Th1) and Th17 phenotypes, TIM-1 expression is increased both intracellularly and on the plasma membrane. However, TIM-1 is also detected on the extracellular surface of the cell membrane in activated T cells, where it may be present in the bent conformation but also in a functional open structure exposing the IgV-like and mucin-like domains. TIM-1 is also implicated in the development of other human pathologies, including polymorphisms that control susceptibility to, and the persistence of, hepatitis C virus, HIV, and cerebral malaria infections. Interestingly, TIM-1 was also proposed to be involved in the development of autoimmune diseases.

图1 TIM蛋白家族结构

图2 TIM-1在T细胞中的表达与转运

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.  

FOR RESEARCH USE ONLY

参考文献：

[1] Evans JP, et al. 2020. Trends Microbiol. 28(3):224-235.

[2] Angiari S, et al. 2014. Trends Mol Med. 20(12):675-84.