T02301 Recombinant human CD30(His Tag)

产品信息(Information)：

背景介绍(Background)：

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008].

CD30 (Ki-1) was discovered in 1982 in Kiel, Germany by Karl Lennert’s group who produced a monoclonal antibody against a Hodgkin lymphoma cell line, naming the unique antigen detected Ki-1. It was later cloned and described as a transmembrane glycoprotein receptor (120kd) of the tumor necrosis factor receptor super family 8 (TNFRSF8). The receptor has intracellular, transmembrane and extracellular domains. Its ligand (CD30L, TNFSF8 or CD153) is a membrane bound cytokine member of the TNF family, and expressed in activated lymphocytes, histiocytes and granulocytes. CD30 mediates its effects through a number of signaling pathways, which in turn produce a survival advantage to the cells on which the protein is overexpressed. Stimulation of CD30 leads to trimerization and signal mediation through tumor necrosis factor receptor-associated proteins (TRAF), to stimulate the nuclear factor-kappa B (NFkB) pathway. CD30 also signals through the mitogen-activated protein kinase (MAPK) pathways, including ERK1 and ERK2, which have diverse anti-apoptotic and pro-survival benefits in neoplastic cells.

图 CD30信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.  

FOR RESEARCH USE ONLY

参考文献：

[1]Xu ML, et al. 2020. Am J Surg Pathol. 44(2):e1-e14.

[2] van der Weyden C A, et al. 2017. Blood Cancer J. 7(9):e603.