T03001 Recombinant human EGFR (His Tag)

产品信息(Information)：

背景介绍(Background)：

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020].

Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase located on chromosome band 7p12. It is a member of the ErbB family of receptors which consists of 4 related tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). The kinase domain of EGFR has 2 lobes, the N-lobe and the C-lobe, that are separated by the ATP binding cleft. The N-lobe of the EGFR kinase domain is mainly formed of β-strands and the regulatory αC-helix, whereas the larger C-lobe is mainly α-helical and contains the activation loop (A-loop). The inactive state of wild-type (WT) EGFR is characterized by an outward rotation of the αC-helix that is stabilized by a helical turn within the N-terminal portion of the A-loop. Upon binding with ligands, EGFR is activated and leads to the excitation of subsequent intracellular signaling pathways, such as the PI3K/Akt and MAPK, which in turn stimulate the transcription factors to drive the associated genes expression which are related with proliferation, angiogenesis, invasion and metastasis. These signalings are involved in the proliferation, differentiation, migration, and apoptosis of certain cells. Consequently, overactivation of EGFR signaling pathways is detected in various malignant tumors, including non-small cell lung cancer (NSCLC), breast cancer, head and neck cancer, colon cancer, ovarian cancer, and the like.

图 EGFR信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.

FOR RESEARCH USE ONLY

参考文献：

[1] Harrison PT, et al. 2020. Semin Cancer Biol. 61:167-179.

[2] Saadeh FS, et al. 2018. Int J Biol Markers. 33(1):22-32.

[3] Liu Q, et al. 2018. Mol Cancer. 17(1):53.