T04501 Recombinant human ROR1-30(His Tag)

产品信息(Information)：

背景介绍(Background)：

This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012].

Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is a member of the receptor tyrosine kinase family, were initially identified in a human neuroblastoma cell line in 1992. ROR1 contains an extracellular section, a transmembrane segment, and a cytoplasmic region. The extracellular part comprises an immunoglobulin-like domain (IG), a cysteine rich domain (CRD), and a Kringle domain (KD). CRD modulates non-canonical Wnt signaling by binding to the ligand Wnt5a. KD mediates the interaction of ROR1 with other receptors such as ROR2. The cytoplasmic section includes a tyrosine kinase domain, two serine/threonine-rich domains, and a proline-rich domain. The proline-rich domain is responsible for the activation of cell migration and proliferation signals by recruiting SH3 domain-containing proteins including HS1, DOCK2, and cortactin. In addition, wnt5a binding to ROR1 induces intracellular signaling, leading to an increased AKT activation that in turn can phosphorylate BMI-1 to promote genomic instability, cancer cell proliferation and drug-resistance. Wnt5a binding to ROR1/FZD complex will activate RhoA via Gα12/13 engagement, resulting in the inhibition of Lats1/2 activity and consequently, YAP/TAZ dephosphorylation and nuclear translocation. Binding of YAP/TAZ to TEADs can induce the transcription of genes involved in stemness and tumorigenesis. Increased YAP/TAZ transcription could, in turn, upregulate ROR1 expression. ROR1 was expressed predominantly during embryogenesis, mainly in the central nervous system, the early limb bud, cartilage condensations, cartilage growth plate, heart, lung, and mesonephros in mice. ROR1 knockout is embryonic lethal, indicating a critical role of ROR1 in embryogenesis. ROR1 is not expressed in most postpartum tissues, except for adipose tissues that highly express ROR1, and some tissues from the endocrine glands, gastrointestinal tract, and immature B lymphocytes.

图1 ROR1结构示意图

图2 ROR1信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.  

FOR RESEARCH USE ONLY

参考文献：

[1] Zhao Y, et al. 2021. Front Oncol. 11:680834.

[2] Karvonen H, et al. 2019. Cells. 8(8):812.