Y00203 Recombinant human BDNF (hFc Tag)

产品信息(Information)：

背景介绍(Background)：

This protein belongs to a member of the nerve growth factor family of proteins. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this protein is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This protein may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]

Brain-derived neurotrophic factor (BDNF) is one of the most distributed and extensively studied neurotrophins in the mammalian brain. BDNF is secreted into the synapse leading to the activation of tropomyosin receptor kinase B (TrkB) and its downstream signaling cascades that contribute to gene transcriptional events critical for synaptic plasticity and cognitive function. BDNF plays an important role in proper growth, development, and plasticity of glutamatergic and GABAergic synapses and through modulation of neuronal differentiation, it influences serotonergic and dopaminergic neurotransmission. BDNF acts as paracrine and autocrine factor, on both pre-synaptic and post-synaptic target sites. BDNF is considered an instructive mediator of functional and structural plasticity in the central nervous system, influencing dendritic spines and, at least in the hippocampus, the adult neurogenesis. Synthesis and maturation of BDNF is a multistage process, involving the formation of several precursor isoforms, including preproBDNF (32–35 kDa), proBDNF (28–32 kDa), and mBDNF (13 kDa). The mature domain of proBDNF interacts preferentially with p75NTR, mediating synaptic pruning in the prenatal brain. ProBDNF, through its pro-domain, can also interact with the sortilin receptor or other vacuolar protein sorting 10 protein (Vps10p). Thus, proBDNF binding to specific receptors triggers signaling pathways, which can determine neuronal fate via promoting their death or survival. The proBDNF/p75NTR/sortilin binding complex initiates signaling cascades leading to the activation of c-Jun amino terminal kinase. This pathway is involved in neuronal apoptosis. High levels of p75NTR expression are detected during brain development and post-traumatic recovery. When mature domain of BDNF binds to p75NTR, the RIP2/TRAF6-mediated pathway is initiated, which leads to NF-kB activation. The activation of NF-kB promotes neuronal survival and maintenance during brain development. In addition, p75NTR interacts also with the Ras homologous protein family. This pathway is reported to regulate neuronal growth cone development and motility.

图 BDNF信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.

FOR RESEARCH USE ONLY

参考文献：

[1] Colucci-D'Amato L, et al. 2020. Int J Mol Sci. 21(20):7777.

[2] Song M, et al. 2017. Mol Psychiatry. 22(10):1370-1375.