Y02201 Recombinant Human IL-4

Characteristics:

Source

Chinese Hamster Ovary cell line

Description

Human Interleukin-4 (His25-Ser153)

Accession # P05112

Predicted molecular mass

15 kDa

Specification:

Appearance

White powder, Colorless clear liquid after reconstitution

Purity

≥95%, by SDS-PAGE (under reducing (R) condition, visualized by Coomassie staining)

Endotoxin

≤10 EU/mg by the LAL method

Activity

Measured in a cell proliferation assay using TF-1 cells, The ED₅₀ for this effect is ≤ 0.2 ng/mL.

Formulation

Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4

Size

50 μg/vial, 1 mg/vial

Handling and Storage:

Reconstitution

It is recommended to redissolve in sterile deionized water.

Shipping

The product is shipped with blue ice.

Storage & Stability

24 months at -20°C to -80°C in lyophilized state

7-10 days at 2°C to 8°C under sterile conditions after reconstitution

6 months at -20°C to -80°C under sterile conditions after reconstitution

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Background:

This protein is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Interleukin-4 (IL-4) is a pleiotropic cytokine mainly produced by activated T lymphocytes, especially Th2 cells, mast cells, and basophils. Although the IL-4 cDNA sequence in human beings contains 153 amino acid residues, it can only produce secreted IL-4 proteins containing 129 amino acid residues. The spherical hydrophobic core of secreted IL-4 contains three disulfide bonds and four α-helices. After the addition of N-chain oligosaccharides, this core produces different molecular weights that include 15, 18, and 19 kDa. Essentially, secreted IL-4 mainly functions as follows: (1) driving Th2 cell differentiation to produce more IL-4, and then promoting the release of other Th2 anti-inflammatory CKs such as IL-5 and IL-13; (2) inhibiting the production of pro-inflammatory CKs such as TNF-α, interferon γ (IFN-γ), and IL-17; and (3) promoting B cell proliferation and differentiation and up-regulating the expression of major histocompatibility complex II (MHC II) molecules, IL-4R and CD23 on B cells. There are two types of IL-4 receptors, Type I and Type II. Type I receptors are a kind of heterodimer receptor that was formed by IL-4 receptor α (IL4Rα) and common r chain (γc) chains and are mainly expressed on the surface of hematopoietic cells. Type II receptors contain the IL4Rα and IL-13 receptor α1 (IL-13Rα1) and are expressed on the surface of non-hematopoietic cells.

Interleukin-13 (IL-13) and IL-4 are the signature cytokines of the type II inflammatory response, and they both signal via the IL-4 receptor (IL-4R) system. IL-4 can signal via both type I and type II IL-4Rs, whereas IL-13 only signals via the type II IL-4R. Additionally, IL-13 can bind to IL-13Rα2, which is thought to be a non-signaling decoy receptor of very high affinity to IL-13. The binding of IL-4 to its receptor leads to the activation of the Janus kinase (JAK) family and the phosphorylation of IL4Rα.

图 IL-4-IL-4R 信号传导

参考文献：

[1] Chen L, et al. 2020. Front Mol Neurosci. 13:554547.

[2] Egholm C, et al. 2019. Front Immunol. 10:2507.

仅供科研或生产使用，不可直接应用于人体。

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