Y03603 Recombinant human R-Spondin 2 (hFc Tag)

产品信息(Information)：

背景介绍(Background)：

This protein is a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015].

R-Spondin 2 (RSPO2) is a member of the R-spondin family, which are secreted activators of the Wnt /β-catenin signaling pathway. RSPO2 is expressed primarily in the lungs and mutations of this protein cause severe defects in the respiratory tract. Loss of RSPO2 reduces Wnt signaling activity in the area of expression, and genetic epistasis was observed when the skeletal phenotype of RSPO2 null mice was exacerbated by crossing to an Lrp6 knockout line, suggesting that the main mechanism of RSPO2 function is through the canonical Wnt pathway. In addition, RSPO2 was found to be upregulated in keratinocytes and fibroblasts obtained from keloid scar, a lesion characterized by extensive dermal fibro-proliferative growth, suggesting that it might have a role in fibrogenesis. RSPO2 may also modulate proliferation and cell death.

R-spondins regulate Wnt /β-catenin signaling process by inhibiting the action of ZNRF3. ZNRF3 acts as a Wnt signaling inhibitor by promoting LRP/Fzd turnover. When RSPO is bound to a LGR receptor, it blocks the turnover promoting activity of ZNRF3.

图 RSPO2参与调控Wnt/β-catenin信号通路

制剂(Formulation)：

Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4. 

质量控制(Quality Control)：

Purity: ≥ 95% as determined by reducing SDS-PAGE.    

Endotoxin: ＜ 0.1 EU/ug  as determined by LAL test.

保存(Storage)：

Use a manual defrost freezer and avoid repeated freeze - thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
6 months, -20 to -70℃ under sterile conditions after reconstitution.   

FOR RESEARCH USE ONLY

参考文献：

[1] Munguía-Reyes A, et al. 2018. Am J Respir Cell Mol Biol. 59(1):65-76.

[2] Knight MN, et al. 2014. Matrix Biol. 37:157-161.

[3] Dong X, et al. 2017. Cancer Letters. 402: 153-165.