VISC2-RB04 重组新冠RBD his标签 Recombinant SARS CoV2 RBD (His Tag)

Source：

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Cat.No.: 

VISC2-RB04

Construction：

Arg319-phe541

Tag:

His Tag

Molecular Mass：

26.20 kDa

Expression Host：

HEK293 cells

Formulation：

Purified, Liquid

Buffer：

Phosphate Buffered Saline,5%mannital,0.05% Tween20, pH7.4

Purification：

>95% pure (SDS-PAGE). 

Storage:

For long term storage, the product should be stored at -20°C or lower.

 

Please avoid repeated freeze-thaw cycles.

 

 

 

Description：

The single-positive-strand RNA genome of SARS-CoV-2, encodes four major structural proteins: spike (S), envelope (E), membrane (M) and nucleocapsid (N). The spike protein comprises an N-terminal (S1) subunit, which contains the roughly 200-residue receptor binding domain (RBD), and a C-terminal subunit (S2), which contains the fusion protein. The RBD of SARS-CoV-2 binds more tightly to the extracellular domain of angiotensin-converting enzyme 2 (ACE2) than the homologous SARS-CoV (2002) RBD. The higher affinity results from sequence changes in RBD and this has been proposed to underlie the higher transmissibility of SARS-CoV-2. The previously determined structures showed that SARS-CoV RBD contains a core structure and a receptor-binding motif (RBM) and that the RBM binds to the outer surface of the claw-like structure of ACE2. Importantly, two virus-binding hot spots on human ACE2 has been identified. A number of naturally selected RBM mutations occurred near these two virus-binding hot spots, and these residues largely determined the host range of SARS-CoV.

 

                

             

               图 SARS-CoV-2 刺突蛋白示意图及人类ACE2与SARS-CoV RBD（2002）复合体三维结构

 

 

 

 

 

 

 

参考文献：

[1] Wan Y, et al. 2020. J Virol. 94(7):e00127-20.

[2] Huo J, et al. 2020. Nat Struct Mol Biol. 27(9):846-854.