A03301 重组扎伊尔埃博拉病毒抗原 Recombinant Ebola Zaire GP1

Source：

Zaire ebolavirus

Cat.No.: 

A03301

Tag:

His Tag

Molecular Mass：

29.14 kDa

Expression Host：

HEK293 cells

Formulation：

Purified, Liquid

Buffer：

Phosphate Buffered Saline,5%mannital,0.05% Tween20, pH7.4

Purification：

>95% pure (SDS-PAGE). 

Storage:

For long term storage, the product should be stored at -20°C or lower.

 

Please avoid repeated freeze-thaw cycles.

 

 

 

Description：

The Ebola virus is an enveloped, non-segmented, negative-strand RNA virus, belonging to the filoviridae family. Totally, five species of the virus have been identified, including Zaire ebolavirus, Sudan ebolavirus(Sudan virus), Bundibugyo ebolavirus (Bundibugyo virus), Taϊ Forest ebolavirus (Taϊ Forest virus), and Reston ebolavirus (Reston virus). The full-length transmembrane glycoprotein (GP1,2), one of the structural proteins encoded by Ebola viruses, is the virus surface protein and forms trimeric spikes that facilitate virus entry by mediating receptor binding and membrane fusion. GP1 and GP2 are the disulfide-linked subunits formed during the processing in the endoplasmic reticulum and Golgi apparatus , which are furin-cleavage products of the full-length GP polyprotein precursor. GP trimerization is mediated through multiple GP1-GP2 and GP2-GP2 contacts. GP1 is responsible for cell attachment, while GP2 is responsible for fusion of viral and host cell membranes. GP1 is composed of a single domain ( ～65 Å×30 Å ×30 Å) arranged in the topology, and can be further subdivided into the (I) base, (II) head and (III) glycan cap regions. GP2 contains the internal fusion loop and the heptad repeat regions, HR1 and HR2.

 

 

图1 埃博拉病毒GP1和GP2示意图

 

 

图2 GP1,2在埃博拉病毒发病机制中的作用模型

 

 

 

 

 

 

 

 

参考文献：

[1] Lee JE, et al, 2008. Nature. 454(7201):177-182.

[2] Ning YJ, et al. 2017. Virologica Sinica. 32(1):3-15.