C03601 肌红蛋白（Myo）单抗（Mouse，包被）

Cat.No.: 

C03601

Source：

Monoclonal Mouse Antibody

Subtype：

mIgG1/mK

Molecular Mass：

150 kDa

Expression Host：

HEK293 cells

Formulation：

Purified, Liquid

Buffer：

Phosphate Buffered Saline,5%mannital,0.05% Tween20, pH7.4

Purification：

>95% pure (SDS-PAGE). 

Storage:

For long term storage, the product should be stored at -20°C or lower.

 

Please avoid repeated freeze-thaw cycles.

 

 

 

Description：

Myoglobin (Mb) is a monomeric 17,800-Da protein, which incorporates a heme group in a hydrophobic crevice during the folding process.It can transport and store oxygen in muscles. Mb is present in oxidative striated muscles and cardiac myocytes as well as in smooth muscle cells. Notably, Mb has also been traced in human tumor-associated mammary cells. Mb can be detected in the serum as early as two hours after myocardial necrosis begins. Mb has low cardiac specificity but high sensitivity, which makes it most useful for ruling out myocardial infarction if the level is normal in the first four to eight hours after the onset of symptoms.

 

 

图1 氧(O2)与肌红蛋白结合的示意图

O2以弯曲的几何形状与铁（FeII）结合，铁（FeII）嵌入原卟啉环中并与近端组氨酸结合，远端组氨酸为结合的O2提供稳定的氢键。

 

 

 

 

图2 肌红蛋白的结构

每个α螺旋用不同的颜色表示，血红素辅基（白色）包含一个与氧结合的中心铁原子（棕色）。

 

 

 

 

 

 

 

 

 

 

参考文献：

[1] Hendgen-Cotta Ulrike B,et al. 2014. Free Radic Biol Med. 73:252-9.

[2] Achar SA, et al. 2005. Am Fam Physician.. 72(1):119-26.

[3] McConnell S C. Hemoglobin switching, thalassemia and sickle cell disease in humanized knockin mice[M]. The University of Alabama at Birmingham, 2011.